Articles published before 2000Articles published before 2000Articles published between 2000 and 2004
Articles published between 2005 and 2009
Publications in 2005
| Author | Title | Year | Journal/Proceedings | Reftype | DOI/URL |
|---|---|---|---|---|---|
| Lentzen, O., Moucheron, C. and Kirsch-De Mesmaeker, A. | Metallotherapeutic drug & Metal-based diagnostic agents
[ BibTeX] |
2005 | , pp. 359-378 | inbook | |
BibTeX:
@inbook{BOOK001,
author = {O. Lentzen and C. Moucheron and Kirsch-De Mesmaeker, A.},
title = {Metallotherapeutic drug & Metal-based diagnostic agents},
publisher = {Wiley},
year = {2005},
pages = {359-378}
}
|
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| Blasius, R., Nierengarten, H., Luhmer, M., Constant, J.-F., Defrancq, E., Dumy, P., van Dorsselaer, A., Moucheron, C. and Kirsch-DeMesmaeker, A. | Photoreaction of [Ru(hat)2 phen]2+ with Guanosine-5'-Monophosphate and DNA: Formation of New Types of Photoadducts | 2005 | Chem. Eur. J. Vol. 11(5), pp. 1507-1517 |
article | DOI URL |
| Abstract: [Ru(hat)2phen]2+ (HAT=1,4,5,8,9,12-hexaazatriphenylene, phen=1,10-phenanthroline) interacts with a good affinity with polynucleotides and DNA by intercalation, despite the presence of a second voluminous ancillary HAT ligand. It photoreacts with guanosine-5′-monophosphate (GMP). From HPLC, ESMS and NMR analyses, it can be concluded that this complex forms photoadducts with GMP. In contrast to the photoadducts isolated with Ru–TAP complexes (TAP=1,4,5,8-tetraazaphenanthrene), the photoadducts with [Ru(hat)2phen]2+ contain a covalent link between the oxygen atom of the guanine unit and a HAT ligand. Formation of oxidised photoadducts and compounds resulting from the addition of two GMP entities to the complex are also detected as side products. In the presence of oligo- and polynucleotides, [Ru(hat)2phen]2+ yields photoadducts when guanine bases are present. | |||||
BibTeX:
@article{PUB0049,
author = {Blasius, Romain and Nierengarten, Hélène and Luhmer, Michel and Constant, Jean-François and Defrancq, Eric and Dumy, Pascal and van Dorsselaer, Alain and Moucheron, Cécile and Kirsch-DeMesmaeker, Andrée},
title = {Photoreaction of [Ru(hat)2 phen]2+ with Guanosine-5'-Monophosphate and DNA: Formation of New Types of Photoadducts},
journal = {Chem. Eur. J.},
publisher = {WILEY-VCH Verlag},
year = {2005},
volume = {11},
number = {5},
pages = {1507-1517},
url = {http://dx.doi.org/10.1002/chem.200400591},
doi = {http://dx.doi.org/10.1002/chem.200400591}
}
|
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| Boisdenghien, A., Moucheron, C. and Kirsch-De Mesmaeker, A. | [Ru(phen)2(PHEHAT)]2+ and [Ru(phen)2(HATPHE)]2+: Two Ruthenium(II) Complexes with the Same Ligands but Different Photophysics and Spectroelectrochemistry | 2005 | Inorg. Chem. Vol. 44(21), pp. 7678-7685 |
article | DOI URL |
| Abstract: The properties of two mononuclear Ru(II) complexes formed with the extended planar ligand PHEHAT depend drastically on the chelation site by the metallic ion. When the chelation takes place on the HAT site of the ligand (case of the novel complex [Ru(phen)2(HATPHE)]2+), the emission behavior is quite similar to that of [Ru(phen)2(HAT)]2+. In contrast, when the chelation is on the phen motif of the ligand (case of [Ru(phen)2(PHEHAT)]2+), the spectroscopic (absorption and emission) and electrochemical data for the complex do not obey the linear spectroelectrochemical correlation and the emission behavior is comparable to that of the extensively studied dppz complex ([Ru(phen/bpy)2(dppz)]2+). Thus, for [Ru(phen)2(PHEHAT)]2+, the emission lifetimes and intensities as a function of temperature exhibit a maximum for nitrile solvents. However, in contrast to the dppz case, at least three different states (two emitting and one dark) participate in the deactivation with different contributions depending on the temperature. These different contributions explain the observed maximum. Moreover, the fact that the solvent is liquid or frozen also influences the nature of the luminescent species. | |||||
BibTeX:
@article{PUB0213,
author = {Boisdenghien, Arnaud and Moucheron, Cécile and Kirsch-De Mesmaeker, Andrée},
title = {[Ru(phen)2(PHEHAT)]2+ and [Ru(phen)2(HATPHE)]2+: Two Ruthenium(II) Complexes with the Same Ligands but Different Photophysics and Spectroelectrochemistry},
journal = {Inorg. Chem.},
year = {2005},
volume = {44},
number = {21},
pages = {7678-7685},
url = {http://pubs.acs.org/doi/abs/10.1021/ic051306f},
doi = {http://dx.doi.org/10.1021/ic051306f}
}
|
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| Garcia-Fresnadillo, D., Lentzen, O., Ortmans, I., Defrancq, E. and Kirsch-De Mesmaeker, A. | Detection of secondary structures in 17-mer Ru(II)-labeled single stranded oligonucleotides from luminescence lifetime studies | 2005 | Dalton Trans.(5), pp. 852-856 | article | DOI |
| Abstract: The emission properties of a non intercalating complex, [Ru(TAP)2(dip)]2+ (TAP = 1,4,5,8-tetraazaphenanthrene; dip = 4,7-diphenyl-1,10-phenanthroline), tethered to 17-mer single-stranded oligodeoxyribonucleotides (ODNs)either in the middle or at the 5-end of the sequence, are determined. The results highlight the fact that the luminescence of this metallic compound is sufficiently sensitive to its microenvironment to probe self-structuration of these short single-stranded ODNs. It is shown that the weighted averaged emission lifetimes (sM) along with the quenching rate constants of luminescence by oxygen reflect particularly well different structures adopted by the different ODNs sequences. The determination of these parameters thus offers an elegant way to examine possible structurations of synthetic single-stranded ODNs that play important roles in biological applications. | |||||
BibTeX:
@article{PUB0606,
author = {D. Garcia-Fresnadillo and O. Lentzen and I. Ortmans and E. Defrancq and Kirsch-De Mesmaeker, A.},
title = {Detection of secondary structures in 17-mer Ru(II)-labeled single stranded oligonucleotides from luminescence lifetime studies},
journal = {Dalton Trans.},
year = {2005},
number = {5},
pages = {852-856},
doi = {http://dx.doi.org/10.1039/b415898a}
}
|
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| Leveque, J., Elias, B., Moucheron, C. and Kirsch-De Mesmaeker, A. | Dendritic Tetranuclear Ru(II) Complexes Based on the Nonsymmetrical PHEHAT Bridging Ligand and Their Building Blocks: Synthesis, Characterization, and Electrochemical and Photophysical Properties | 2005 | Inorg. Chem. Vol. 44(2), pp. 393-400 |
article | DOI URL |
| Abstract: Dinuclear and tetranuclear Ru(II) compounds 1, 2, 3, and 4 based on the PHEHAT ligand (PHEHAT = 1,10-phenanthrolino[5,6-b]-1,4,5,8,9,12-hexaazatriphenylene) are prepared and characterized on the basis of the data for other related mononuclear species. Their electrochemical and spectroscopic behaviors are discussed. The nonspectroelectrochemical correlation obtained for 1, 2, 3, and 4 is explained on the basis of these data. From the behavior in emission, it is concluded that the internal energy transfer takes place from the core to the peripheral metallic units in 3 and 4. | |||||
BibTeX:
@article{PUB1095,
author = {Leveque, Julien and Elias, Benjamin and Moucheron, Cécile and Kirsch-De Mesmaeker, Andrée},
title = {Dendritic Tetranuclear Ru(II) Complexes Based on the Nonsymmetrical PHEHAT Bridging Ligand and Their Building Blocks: Synthesis, Characterization, and Electrochemical and Photophysical Properties},
journal = {Inorg. Chem.},
year = {2005},
volume = {44},
number = {2},
pages = {393-400},
note = {PMID: 15651886},
url = {http://pubs.acs.org/doi/abs/10.1021/ic048941q},
doi = {http://dx.doi.org/10.1021/ic048941q}
}
|
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Publications in 2006
| Author | Title | Year | Journal/Proceedings | Reftype | DOI/URL |
|---|---|---|---|---|---|
| Elias, B. and Kirsch-De Mesmaeker, A. | Photo-reduction of polyazaaromatic Ru(II) complexes by biomolecules and possible applications | 2006 | Coord. Chem. Rev. Vol. 250(13–14), pp. 1627-1641 |
article | DOI URL |
| Abstract: An important property of the Ru(II) complexes that contain polyazaaromatic ligands such as TAP (1,4,5,8-tetraazaphenanthrene) and HAT (1,4,5,8,9,12-hexaazatriphenylene) is the very high oxidation potential of the 3MLCT (Metal to Ligand Charge Transfer) state. Therefore, under illumination, they are able to abstract an electron even from rather poor electron donors. In this work, we show how it is possible to take advantage of this property for applications of photo-induced electron transfer between these excited complexes and biopolymers such as DNA, oligonucleotides and oligopeptides. One of the consequences of the charge transfer processes is photocleavage of plasmid DNA, demonstrated some years ago by agarose gel electrophoresis and more recently detected and quantified by AFM. Another effect of the photo-induced electron transfer is the formation of photo-adduct(s) of the metal complex with the guanine units of oligonucleotides or with the tryptophan moieties of oligopeptides. In the photo-adducts with the guanine nucleobases, a covalent bond is formed between one of the polyazaaromatic ligands and the guanine base without destruction of the ligands sphere around the metal ion. Interestingly, the formation of these photo-adducts can be exploited in order to photo-crosslink two single-stranded oligonucleotides. Such photo-crosslinking via the metal complexes could lead to applications in biological diagnostic tests and possibly in vivo in the area of gene phototherapy. |
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BibTeX:
@article{PUB0006,
author = {Benjamin Elias and Kirsch-De Mesmaeker, Andrée},
title = {Photo-reduction of polyazaaromatic Ru(II) complexes by biomolecules and possible applications},
journal = {Coord. Chem. Rev.},
year = {2006},
volume = {250},
number = {13–14},
pages = {1627-1641},
url = {http://www.sciencedirect.com/science/article/pii/S001085450500278X},
doi = {http://dx.doi.org/10.1016/j.ccr.2005.11.011}
}
|
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| Pierard, F. and Kirsch-De Mesmaeker, A. | Bifunctional transition metal complexes as nucleic acid photoprobes and photoreagents | 2006 | Inorg. Chem. Commun. Vol. 9(1), pp. 111-126 |
article | DOI URL |
| Abstract: Transition metal complexes that bind to DNA have been the focus of extensive research aimed at increasing the understanding of genetic information processing and at developing ways to interfere with these mechanisms. Beside the continuous development of “dark reacting” artificial metallonucleases and metal-based chemotherapeutics such as cisplatin, photo-active octahedral metal complexes have been successfully used as DNA luminescent probes and light-driven reactive agents during the last decades. A recent emerging trend to improve their potential as molecular tools for studies of the genetic material is the design of bifunctional assemblies where the photo-active metal center is tethered through a flexible linker to a nucleic acid recognition or reactive moiety. This mini-review highlights some recent progresses in developing such bifunctional complexes through derivatization strategies ranging from the design of dimeric complexes and chromophore-quencher systems to the development of biotin–metal complex conjugates for avidin-mediated detection of biomolecules. | |||||
BibTeX:
@article{PUB0130,
author = {Frédéric Pierard and Kirsch-De Mesmaeker, Andrée},
title = {Bifunctional transition metal complexes as nucleic acid photoprobes and photoreagents},
journal = {Inorg. Chem. Commun.},
year = {2006},
volume = {9},
number = {1},
pages = {111-126},
url = {http://www.sciencedirect.com/science/article/pii/S1387700305003412},
doi = {http://dx.doi.org/10.1016/j.inoche.2005.10.016}
}
|
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| Uji-i, H., Foubert, P., De Schryver, F.C., De Feyter, S., Gicquel, E., Etoc, A., Moucheron, C. and Kirsch-De Mesmaeker, A. | [Ru(TAP)3]2+-Photosensitized DNA Cleavage Studied by Atomic Force Microscopy and Gel Electrophoresis: A Comparative Study | 2006 | Chem. Eur. J. Vol. 12(3), pp. 758-762 |
article | DOI URL |
| Abstract: Topological modifications of plasmid DNA adsorbed on a variety of surfaces were investigated by using atomic force microscopy (AFM). On mica modified with 3-aminopropyltriethoxysilane (APS) or poly-L-lysine, the interaction between the plasmid DNA and the surface “freezes” the plasmid DNA conformation deposited from solution, and the AFM images resemble the projection of the three-dimensional conformation of the plasmid DNA in solution. Modified mica with low concentrations of Mg2+ leads to a decrease in the interaction strength between plasmid DNA and the substrate, and the AFM images reflect the relaxed or equilibrium conformation of the adsorbed plasmid DNA. Under these optimized deposition conditions, topological modifications of plasmid DNA were produced under irradiation in the presence of [Ru(TAP)3]2+ (TAP=1,4,5,8-tetraazaphenanthrene), which is a non-intercalating complex, and were followed as a function of illumination time. The observed structural changes correlate well with the conversion of the supercoiled covalently closed circular form (ccc form) into the open circular form (oc form), induced by a single-strand photocleavage. The AFM results obtained after fine-tuning of the plasmid DNA–substrate interaction compare well with those observed from gel electrophoresis, indicating that under the appropriate deposition conditions, AFM is a reliable technique to investigate irradiation-induced topological changes in plasmid DNA. | |||||
BibTeX:
@article{PUB0238,
author = {Uji-i, Hiroshi and Foubert, Philippe and De Schryver, Frans C. and De Feyter, Steven and Gicquel, Etienne and Etoc, Ariane and Moucheron, Cécile and Kirsch-De Mesmaeker, Andrée},
title = {[Ru(TAP)3]2+-Photosensitized DNA Cleavage Studied by Atomic Force Microscopy and Gel Electrophoresis: A Comparative Study},
journal = {Chem. Eur. J.},
publisher = {WILEY-VCH Verlag},
year = {2006},
volume = {12},
number = {3},
pages = {758-762},
url = {http://dx.doi.org/10.1002/chem.200500419},
doi = {http://dx.doi.org/10.1002/chem.200500419}
}
|
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| Bijeire, L., Elias, B., Souchard, J., Gicquel, E., Moucheron, C., Kirsch-De Mesmaeker, A. and Vicendo, P. | Photoelectron Transfer Processes with Ruthenium(II) Polypyridyl Complexes and Cu/Zn Superoxide Dismutase | 2006 | Biochemistry Vol. 45(19), pp. 6160-6169 |
article | DOI |
| Abstract: The processes that are photoinduced by [Ru(bpz)3]2+ (bpz = 2,2‘-bipyrazyl) in the presence of Cu/Zn superoxide dismutase (Cu/Zn SOD) are investigated by laser flash photolysis and electron paramagnetic resonance (EPR) spectroscopy; they are compared to those of the system [Ru(bpy)32+−Cu/Zn SOD]. Although the mechanism is complicated, primary and secondary reactions can be evidenced. First, the excited [Ru(bpz)3]2+ complex is quenched reductively by Cu/Zn SOD with the production of a reduced complex and an oxidized enzyme. The oxidation site of Cu/Zn SOD is proposed to correspond to amino acids located on the surface of the protein. Afterward and only when this reductive electron transfer to the excited complex has produced enough oxidized protein, another electron-transfer process can be evidenced. In this case, however, the charge-transfer process takes place in the other direction, i.e., from the excited complex to the CuII center of the SOD with the formation of RuIII and CuI species. This proposed mechanism is supported by the fact that [Ru(bpy)3]2+, which is less photo-oxidizing than [Ru(bpz)3]2+, exhibits no photoreaction with Cu/Zn SOD. Because RuIII species are generated as intermediates with [Ru(bpz)3]2+, they are proposed to be responsible for the enhancement of [poly(dG-dC)]2 and [poly(dA-dT)]2 oxidation observed when Cu/Zn SOD is added to the [Ru(bpz)3]2+−DNA system. | |||||
BibTeX:
@article{PUB0617,
author = {L. Bijeire and B. Elias and J.P. Souchard and E. Gicquel and C. Moucheron and Kirsch-De Mesmaeker, Andrée and P. Vicendo},
title = {Photoelectron Transfer Processes with Ruthenium(II) Polypyridyl Complexes and Cu/Zn Superoxide Dismutase},
journal = {Biochemistry},
year = {2006},
volume = {45},
number = {19},
pages = {6160-6169},
doi = {http://dx.doi.org/10.1021/bi060005u}
}
|
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Publications in 2007
| Author | Title | Year | Journal/Proceedings | Reftype | DOI/URL |
|---|---|---|---|---|---|
| Villien, M., Deroo, S., Gicquel, E., Defrancq, E., Moucheron, C., Kirsch-De Mesmaeker, A. and Dumy, P. | The oxime bond formation as an efficient tool for the conjugation of ruthenium complexes to oligonucleotides and peptides | 2007 | Tetrahedron Vol. 63(46), pp. 11299-11306 |
article | DOI URL |
| Abstract: A convenient method for the conjugation of ruthenium complex on oligonucleotides and peptides through chemoselective oxime linkage is reported. Novel Ru(II) complexes sustaining an aminooxy containing ligand were prepared and efficiently coupled with the oligonucleotides and peptides functionalized with the complementary reactive aldehyde group. The method described herein could be a useful tool for preparing a broad range of metal complex–oligonucleotide and peptide conjugates. | |||||
BibTeX:
@article{PUB0013,
author = {Mathilde Villien and Stéphanie Deroo and Etienne Gicquel and Eric Defrancq and Cécile Moucheron and Kirsch-De Mesmaeker, Andrée and Pascal Dumy},
title = {The oxime bond formation as an efficient tool for the conjugation of ruthenium complexes to oligonucleotides and peptides},
journal = {Tetrahedron},
year = {2007},
volume = {63},
number = {46},
pages = {11299-11306},
url = {http://www.sciencedirect.com/science/article/pii/S0040402007015281},
doi = {http://dx.doi.org/10.1016/j.tet.2007.08.088}
}
|
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| Elias, B., Herman, L., Moucheron, C. and Kirsch-De Mesmaeker, A. | Dinuclear Ru(II)PHEHAT and -TPAC Complexes: Effects of the Second Ru(II) Center on Their Spectroelectrochemical Properties | 2007 | Inorg. Chem. Vol. 46(12), pp. 4979-4988 |
article | DOI URL |
| Abstract: Four novel dinuclear RuII compounds and, for comparison purposes, two corresponding mononuclear complexes containing the PHEHAT or TPAC ligand (PHEHAT = 1,10-phenanthrolino[5,6-b]-1,4,5,8,9,12-hexaazatriphenylene and TPAC = tetrapyrido[3,2-a:2‘,3‘-c:3‘ ‘,2‘ ‘-h:2‘ ‘‘,3‘ ‘‘-j]acridine) have been synthesized and characterized. Conclusions on the effects of dinucleation of these two bridging ligands can be drawn only for the compounds for which the results demonstrate that the bridging ligand is involved in the first electrochemical reduction and lowest emission energy. The behavior of these complexes, which is not always predictable, is discussed, and the differences are highlighted in this work. Interestingly, all of the compounds are luminescent except one dinuclear species, [(phen)2Ru-μ-PHEHAT-Ru(TAP)2]4+, which does not luminesce in MeCN and BuCN at room temperature. | |||||
BibTeX:
@article{PUB0157,
author = {Elias, Benjamin and Herman, Leslie and Moucheron, Cécile and Kirsch-De Mesmaeker, Andrée},
title = {Dinuclear Ru(II)PHEHAT and -TPAC Complexes: Effects of the Second Ru(II) Center on Their Spectroelectrochemical Properties},
journal = {Inorg. Chem.},
year = {2007},
volume = {46},
number = {12},
pages = {4979-4988},
url = {http://pubs.acs.org/doi/abs/10.1021/ic070224j},
doi = {http://dx.doi.org/10.1021/ic070224j}
}
|
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| Herman, L., Elias, B., Pierard, F., Moucheron, C. and Kirsch-De Mesmaeker, A. | Effects of Protonation on the Spectroscopic Properties of Tetrapyridoacridine (TPAC) Mono- and Dinuclear Ru(II) Complexes in Their Ground and 3MLCT Excited States | 2007 | J. Phys. Chem. A Vol. 111(39), pp. 9756-9763 |
article | DOI URL |
| Abstract: The spectroscopic behavior of mono- and dinuclear Ru(II) complexes (P, T, PP and TT, Figure 1) that contain the extended planar ligand tetrapyrido[3,2-a:2‘,3‘-c:3‘ ‘,2‘ ‘-h:2‘ ‘‘,3‘ ‘‘-j]acridine (TPAC) and either 1,10-phenanthroline (phen) or 1,4,5,8-tetraazaphenanthrene (tap) as ancillary ligands is examined in water and as a function of the pH. These four complexes luminesce in aqueous solution. The analyses of the data in absorption lead to the pKa values in the ground state, and the data in emission show that the excited 3MLCT states are much more basic than the ground state. When the complex contains tap ligands (T and TT), a decrease in pH transforms the luminescent excited basic form into another luminescent excited protonated species, which emits more bathochromically. In contrast, with phen ancillary ligands (P and PP), the protonated excited state does not luminesce. The rate constant of first protonation of the 3MLCT state is diffusion controlled, except for the dinuclear PP complex, whose protonation takes place on the nitrogen of the acridine motif. For P, in which the protonation process is the fastest, it would take place on the nitrogen atoms of the nonchelated phen moiety of the TPAC ligand. These results allow also us to gain information on the localization of the excited electron in the 1MLCT state populated upon absorption as well as in the relaxed 3MLCT emissive state. Moreover as these complexes are interesting for their study with DNA, it can be concluded from these data that a portion of the excited species in interaction with DNA will be protonated. | |||||
BibTeX:
@article{PUB0158,
author = {Herman, Leslie and Elias, Benjamin and Pierard, Frédéric and Moucheron, Cécile and Kirsch-De Mesmaeker, Andrée},
title = {Effects of Protonation on the Spectroscopic Properties of Tetrapyridoacridine (TPAC) Mono- and Dinuclear Ru(II) Complexes in Their Ground and 3MLCT Excited States},
journal = {J. Phys. Chem. A},
year = {2007},
volume = {111},
number = {39},
pages = {9756-9763},
url = {http://pubs.acs.org/doi/abs/10.1021/jp072782p},
doi = {http://dx.doi.org/10.1021/jp072782p}
}
|
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| Deroo, S., Toncheva, V., Defrancq, E., Moucheron, C., Schacht, E. and Kirsch-De Mesmaeker, A. | Photo-Cross-Linking between Polymers Derivatized with Photoreactive Ruthenium-1,4,5,8-Tetraazaphenanthrene Complexes and Guanine-Containing Oligonucleotides | 2007 | Biomacromolecules Vol. 8(11), pp. 3503-3510 |
article | DOI URL |
| Abstract: We have shown previously that complexes containing 1,4,5,8-tetraazaphenanthrene (TAP) ligands are able to form photoadducts with the guanine bases of DNA and oligonucleotides. In this work, we have exploited this specific photoreaction for carrying out photo-cross-linkings between guanine-containing oligonucleotides (G-ODNs) and biodegradable polymers derivatized with the photoreactive Ru(II) compounds. The aim in the future is to use these polymer conjugates as vectorizing agents of the metallic compounds inside the cells. Thus, photooxidizing Ru(II) complexes such as [Ru(TAP)3]2+ and [Ru(TAP)2phen]2+ (phen = 1,10-phenanthroline) have been derivatized by an oxyamine function to attach them, via an oxime ether linkage, to a soluble 6 or 80 kDa poly-[N-(2-hydroxyethyl)-l-glutamine] polymer that contains pendent aldehyde groups. It is demonstrated that the resulting Ru-labeled polymers exhibit photophysical properties and a photochemistry that are comparable with those of the free, nonattached complexes. The photo-cross-linkings with the G-ODNs are clearly detected by gel electrophoresis with the 6 kDa Ru conjugates upon illumination. | |||||
BibTeX:
@article{PUB0189,
author = {Deroo, Stéphanie and Toncheva, Veska and Defrancq, Eric and Moucheron, Cécile and Schacht, Etienne and Kirsch-De Mesmaeker, Andrée},
title = {Photo-Cross-Linking between Polymers Derivatized with Photoreactive Ruthenium-1,4,5,8-Tetraazaphenanthrene Complexes and Guanine-Containing Oligonucleotides},
journal = {Biomacromolecules},
year = {2007},
volume = {8},
number = {11},
pages = {3503-3510},
url = {http://pubs.acs.org/doi/abs/10.1021/bm700647b},
doi = {http://dx.doi.org/10.1021/bm700647b}
}
|
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| Bouffier, L., Demeunynck, M., Dumy, P., Moucheron, C. and Kirsch-De Mesmaeker, A. | Dipyrido[3,2-a:2′,3′-c]quinolino[3,2-j]phenazine (dpqp-OH) – Synthesis, characterization and DNA interaction of the corresponding Ru(II) complex | 2007 | Inorg. Chim. Acta Vol. 360(10), pp. 3162-3168 |
article | DOI URL |
| Abstract: A mononuclear Ru(II) complex based on a new heptacyclic ligand (dpqp) has been prepared and characterized by NMR spectroscopy, ES mass spectrometry and electrochemistry. It forms dimers and aggregates of up to seven complex units in CH3CN solution observed by ESMS. The monomer has an extremely weak luminescence in water or even in organic solvent probably due to the existence of a low lying π–π∗ excited state centered on the heptacycle. In spite of the strong interaction of the complex with DNA, its luminescence is not enhanced by the DNA microenvironment. | |||||
BibTeX:
@article{PUB0239,
author = {Laurent Bouffier and Martine Demeunynck and Pascal Dumy and Cécile Moucheron and Kirsch-De Mesmaeker, Andrée},
title = {Dipyrido[3,2-a:2′,3′-c]quinolino[3,2-j]phenazine (dpqp-OH) – Synthesis, characterization and DNA interaction of the corresponding Ru(II) complex},
journal = {Inorg. Chim. Acta},
year = {2007},
volume = {360},
number = {10},
pages = {3162-3168},
url = {http://www.sciencedirect.com/science/article/pii/S0020169307001739},
doi = {http://dx.doi.org/10.1016/j.ica.2007.03.017}
}
|
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| Boisdenghien, A., Leveque, J., Moucheron, C. and Kirsch-De Mesmaeker, A. | Synthesis, characterization, electrochemical and photophysical properties of elbow-shaped trinuclear Ru(II) complexes, building blocks for novel supramolecular constructions | 2007 | Dalton Trans., pp. 1705-1712 | article | DOI URL |
| Abstract: Three trinuclear elbow-shaped Ru() complexes based on the non symmetrical bridging PHEHAT ligand (PHEHAT = 1,10-phenanthrolino[5,6-b]-1,4,5,8,9,12-hexaazatriphenylene) have been prepared and characterized by NMR, electrochemistry, absorption and emission spectroscopy. It is shown that the dichloro trinuclear complex should behave as an excellent precursor for the synthesis of larger species. Indeed, it reacts easily with 1,10-phenanthroline (phen) and 1,4,5,8-tetraazaphenanthrene (TAP) and leads to the trinuclear compounds and , respectively. The electrochemical and emission studies indicate that for and , there is an intramolecular energy transfer from the center to the periphery of the elbow-shaped trinuclear complex, whereas for complex the energy transfer takes place in the other direction. | |||||
BibTeX:
@article{PUB0253,
author = {Boisdenghien, Arnaud and Leveque, Julien and Moucheron, Cecile and Kirsch-De Mesmaeker, Andrée},
title = {Synthesis, characterization, electrochemical and photophysical properties of elbow-shaped trinuclear Ru(II) complexes, building blocks for novel supramolecular constructions},
journal = {Dalton Trans.},
publisher = {The Royal Society of Chemistry},
year = {2007},
pages = {1705-1712},
url = {http://dx.doi.org/10.1039/B618645A},
doi = {http://dx.doi.org/10.1039/B618645A}
}
|
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| Claessens, N., Pierard, F., Bresson, C., Moucheron, C. and Kirsch-De Mesmaeker, A. | Optically active Ru(II) complexes with a chiral Tröger's base ligand and their interactions with DNA | 2007 | J. Inorg. Biochem. Vol. 101(7), pp. 987-996 |
article | DOI |
| Abstract: Three stereoisomers of a Ru(II) complex bearing a chiral bis-phenanthroline Tr�ger's base analogue, TBphen2 (1), have been isolated from the reaction of the enantiomerically pure precursor complex K- (or D-) cis-$Ru(phen)2(py)2 2+$ (phen = 1,10-phenanthroline, py = pyridine) with the racemic mixture of 1. Each stereoisomer of $Ru(phen)2TBphen2 2+$ (2) has been characterized by 1H NMR and CD spectroscopy. Electrochemical studies revealed that the redox properties of 2 are not influenced by the stereochemistry, however, the electrochemical oxidation of the metallic center is irreversible because of the diazocine bridge of the TBphen2 ligand. Steady-state emission measurements in the presence of calf thymus DNA showed that the DNA binding of $Ru(phen)2TBphen2 2+$ depends on the stereoisomer and is mainly controlled by the absolute configuration of the metal center of the complex. The affinity constant for the stereoisomer D-S-2 is 102 higher than that for K-S-2 and rac-$Ru(phen)3 2+$. |
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BibTeX:
@article{PUB0613,
author = {N. Claessens and F. Pierard and C. Bresson and C. Moucheron and Kirsch-De Mesmaeker, A.},
title = {Optically active Ru(II) complexes with a chiral Tröger's base ligand and their interactions with DNA},
journal = {J. Inorg. Biochem.},
year = {2007},
volume = {101},
number = {7},
pages = {987-996},
doi = {http://dx.doi.org/10.1016/j.jinorgbio.2007.03.008}
}
|
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| Laza, D., Nys, B., Kinder, J.D., Kirsch-De Mesmaeker, A. and Moucheron, C. | Development of a Quantitative LC-MS/MS Method for the Analysis of Common Propellant Powder Stabilizers in Gunshot Residue | 2007 | J. Forensic Sci. Vol. 52(4), pp. 842-850 |
article | DOI URL |
| Abstract: Abstract: In traditional scanning electron microscopy/energy dispersive X-ray analysis of gunshot residue (GSR), one has to cope more and more frequently with limitations of this technique due to the use of lead-free ammunition or ammunition lacking heavy metals. New methods for the analysis of the organic components of common propellant powder stabilizers were developed based on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). A multiple reactions monitoring scanning method was created for the screening of akardite II, ethylcentralite, diphenylamine, methylcentralite, N-nitrosodiphenylamine, 2-nitrodiphenylamine, and 4-nitrodiphenylamine, present in standards mixtures. Five out of seven of these target compounds can be selectively identified and distinguished from the two others with a high accuracy. Samples from the hands of a shooter were collected by swabbing and underwent solid phase extraction prior to analysis. Detection limits ranging from 5 to 115 μg injected were achieved. Results from several firing trials show that the LC-MS/MS method is suitable for the detection of stabilizers in samples collected following the firing of 9 mm Para ammunitions. | |||||
BibTeX:
@article{PUB1569,
author = {Laza, Désiré and Nys, Bart and Kinder, Jan De and Kirsch-De Mesmaeker, Andrée and Moucheron, Cécile},
title = {Development of a Quantitative LC-MS/MS Method for the Analysis of Common Propellant Powder Stabilizers in Gunshot Residue},
journal = {J. Forensic Sci.},
publisher = {Blackwell Publishing Ltd},
year = {2007},
volume = {52},
number = {4},
pages = {842--850},
url = {http://dx.doi.org/10.1111/j.1556-4029.2007.00490.x},
doi = {http://dx.doi.org/10.1111/j.1556-4029.2007.00490.x}
}
|
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Publications in 2008
| Author | Title | Year | Journal/Proceedings | Reftype | DOI/URL |
|---|---|---|---|---|---|
| Defrancq, E., Kirsch-De Mesmaeker, A., Moucheron, C. and Rickling, S. | Photoreactive Ru(II) complexes anchored on oligonucleotides, method for obtaining and use thereof | 2008 | (EP1970378 (A1)) | patent | URL |
| Abstract: The present invention is related to photoreactive Ru (II) complexes anchored on a G-containing oligonucleotide (Formula I), the method for obtaining them and their use, especially for targeting specific nucleotide sequences, to avoid their expression in prokariote or eukaryote cells (especially in bacterial cells, plant cells, animal cells, fungi cells, including yeast cells). | |||||
BibTeX:
@patent{PAT001,
author = {E. Defrancq and Kirsch-De Mesmaeker, A. and C. Moucheron and S. Rickling},
title = {Photoreactive Ru(II) complexes anchored on oligonucleotides, method for obtaining and use thereof},
year = {2008},
number = {EP1970378 (A1)},
url = {http://ep.espacenet.com/PUB0licationDetails/biblio?CC=EP&NR=1970378A1&KC=A1&FT=D&date=20080917&DB=ep.espacenet.com&locale=en_EP}
}
|
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| Herman, L., Ghosh, S., Defrancq, E. and Kirsch-De Mesmaeker, A. | Ru(II) complexes and light: molecular tools for biomolecules | 2008 | J. Phys. Org. Chem. Vol. 21(7-8), pp. 670-681 |
article | DOI URL |
| Abstract: Ruthenium(II) (Ru(II)) complexes are the focus of numerous research works with various applications mainly due to their attractive photophysical and photochemical properties. In biological research areas, in particular, they are developed as efficient photoprobes or photosensors of biological molecules. More interestingly specific Ru(II) complexes bearing π-deficient ligands such as TAP = 1,4,5,8-tetraazaphenanthrene, bpz = 2,2′-bipyrazyl or HAT = 1,4,5,8,9,12-hexaazatriphenylene exhibit interesting photoreactivity with biomolecules (DNA, polypeptides). The photoreactions are initiated by a photo-electron transfer from the biomolecule to the excited metal complex. A back electron transfer (BET) succeeds this primary process. However in competition with this BET, a DNA photocleavage and/or formation of adducts of the complex with guanine units of DNA or with tryptophan (Trp) residues of polypeptides can take place. In this review we highlight the studies of these photo-adducts carried out by our laboratories, and connected to some applications. Copyright © 2008 John Wiley & Sons, Ltd. | |||||
BibTeX:
@article{PUB0007,
author = {Herman, Leslie and Ghosh, Sumana and Defrancq, Eric and Kirsch-De Mesmaeker, Andrée},
title = {Ru(II) complexes and light: molecular tools for biomolecules},
journal = {J. Phys. Org. Chem.},
publisher = {John Wiley & Sons, Ltd.},
year = {2008},
volume = {21},
number = {7-8},
pages = {670-681},
url = {http://dx.doi.org/10.1002/poc.1355},
doi = {http://dx.doi.org/10.1002/poc.1355}
}
|
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| Elias, B., Creely, C., Doorley, G.W., Feeney, M.M., Moucheron, C., Kirsch-De Mesmaeker, A., Dyer, J., Grills, D.C., George, M.W., Matousek, P., Parker, A.W., Towrie, M. and Kelly, J.M. | Photooxidation of Guanine by a Ruthenium Dipyridophenazine Complex Intercalated in a Double-Stranded Polynucleotide Monitored Directly by Picosecond Visible and Infrared Transient Absorption Spectroscopy | 2008 | Chem. Eur. J. Vol. 14(1), pp. 369-375 |
article | DOI URL |
| Abstract: Transient species formed by photoexcitation (400 nm) of [Ru(dppz)(tap)2]2+ (1) (dppz=dipyrido[3,2-a:2′,3′-c]phenazine; tap=1,4,5,8-tetraazaphenanthrene) in aqueous solution and when intercalated into a double-stranded synthetic polynucleotide, [poly(dG-dC)]2, have been observed on a picosecond timescale by both visible transient absorption (allowing monitoring of the metal complex intermediates) and transient infrared (IR) absorption spectroscopy (allowing direct study of the DNA nucleobases). By contrast with its behavior when free in aqueous solution, excitation of 1 when bound to [poly(dG-dC)]2 causes a strong increase in absorbance at 515 nm due to formation of the reduced complex [Ru(dppz)(tap)2]+ (rate constant=(2.0±0.2)×109 s−1). The subsequent reformation of 1 proceeds with a rate constant of (1.1±0.2)×108 s−1. When the process is carried out in D2O, the rates of formation and removal of [Ru(dppz)(tap)2]+ are reduced (rate constants (1.5±0.3)×109 and (0.7±0.2)×108 s−1 respectively) consistent with proton-coupled electron transfer processes. Picosecond transient IR measurements in the 1540–1720 cm−1 region in D2O solution confirm that the reduction of 1 intercalated into [poly(dG-dC)]2 is accompanied by bleaching of IR ground-state bands of guanine (1690 cm−1) and cytosine (1656 cm−1), each with similar rate constants. | |||||
BibTeX:
@article{PUB0056,
author = {Elias, Benjamin and Creely, Caitriona and Doorley, Gerard W. and Feeney, Martin M. and Moucheron, Cécile and Kirsch-De Mesmaeker, Andrée and Dyer, Joanne and Grills, David C. and George, Michael W. and Matousek, Pavel and Parker, Anthony W. and Towrie, Michael and Kelly, John M.},
title = {Photooxidation of Guanine by a Ruthenium Dipyridophenazine Complex Intercalated in a Double-Stranded Polynucleotide Monitored Directly by Picosecond Visible and Infrared Transient Absorption Spectroscopy},
journal = {Chem. Eur. J.},
publisher = {WILEY-VCH Verlag},
year = {2008},
volume = {14},
number = {1},
pages = {369-375},
url = {http://dx.doi.org/10.1002/chem.200700564},
doi = {http://dx.doi.org/10.1002/chem.200700564}
}
|
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Publications in 2009
| Author | Title | Year | Journal/Proceedings | Reftype | DOI/URL |
|---|---|---|---|---|---|
| Le Gac, S., Rickling, S., Gerbaux, P., Defrancq, E., Moucheron, C. and Kirsch-De Mesmaeker, A. | A Photoreactive Ruthenium(II) Complex Tethered to a Guanine-Containing Oligonucleotide: A Biomolecular Tool that Behaves as a “Seppuku Molecule”
[BibTeX] |
2009 | Angew. Chem., Int. Ed. Vol. 48(6), pp. 1122-1125 |
article | DOI URL |
BibTeX:
@article{PUB0028,
author = {Le Gac, S. and S. Rickling and P. Gerbaux and E. Defrancq and C. Moucheron and Kirsch-De Mesmaeker, A.},
title = {A Photoreactive Ruthenium(II) Complex Tethered to a Guanine-Containing Oligonucleotide: A Biomolecular Tool that Behaves as a “Seppuku Molecule”},
journal = {Angew. Chem., Int. Ed.},
publisher = {WILEY-VCH Verlag},
year = {2009},
volume = {48},
number = {6},
pages = {1122-1125},
url = {http://dx.doi.org/10.1002/anie.200804503},
doi = {http://dx.doi.org/10.1002/anie.200804503}
}
|
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| Deroo, S., Le Gac, S., Ghosh, S., Villien, M., Gerbaux, P., Defrancq, E., Moucheron, C., Dumy, P. and Kirsch-De Mesmaeker, A. | Oligonucleotide Duplexes with Tethered Photoreactive Ruthenium(II) Complexes: Influence of the Ligands and Their Linker on the Photoinduced Electron Transfer and Crosslinking Processes of the Two Strands | 2009 | Eur. J. Inorg. Chem. Vol. 2009(4), pp. 524-532 |
article | DOI URL |
| Abstract: The photoreactivity of new RuII-oligonucleotide conjugates is investigated in the presence of their complementary strands. The goal is to determine the origins of different effects of parameters that control the photocrosslinking process of the two strands. Therefore, two RuII compounds, either [Ru(tap)3]2+or [Ru(tap)2phen]2+ (tap = 1,4,5,8-tetraazaphenanthrene, phen = 1,10-phenanthroline) with different oxidation powers, were tethered with different linkers to either the 5′- or 3′-phosphate end of the probe strand before hybridization with the complementary strand. These systems were studied by time-resolved emission spectroscopy, UV/Vis absorption experiments, PAGE and MS (ESI) analyses. The best yields of photocrosslinking (45 %) obtained with [Ru(tap)3]2+ tethered to the 3′-position are due to (i) a higher oxidation power of the complex and (ii) its attachment at the 3′-position. Indeed, this tethering favours the interaction of the Ru compound with the duplex and, therefore, inhibits its photodechelation. This work allows better design of sequence-specific DNA photodamaging agents prior to biological applications.(© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009) | |||||
BibTeX:
@article{PUB0032,
author = {Deroo, Stéphanie and Le Gac, Stéphane and Ghosh, Sumana and Villien, Mathilde and Gerbaux, Pascal and Defrancq, Eric and Moucheron, Cécile and Dumy, Pascal and Kirsch-De Mesmaeker, Andrée},
title = {Oligonucleotide Duplexes with Tethered Photoreactive Ruthenium(II) Complexes: Influence of the Ligands and Their Linker on the Photoinduced Electron Transfer and Crosslinking Processes of the Two Strands},
journal = {Eur. J. Inorg. Chem.},
publisher = {WILEY-VCH Verlag},
year = {2009},
volume = {2009},
number = {4},
pages = {524-532},
url = {http://dx.doi.org/10.1002/ejic.200801083},
doi = {http://dx.doi.org/10.1002/ejic.200801083}
}
|
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| Moucheron, C. | From cisplatin to photoreactive Ru complexes: targeting DNA for biomedical applications | 2009 | New J. Chem. Vol. 33, pp. 235-245 |
article | DOI URL |
| Abstract: Since the discovery of cisplatin, the search for therapeutic agents based on other metallic compounds developed rapidly thanks to the versatility of coordination chemistry. This short review focuses on advances in research for new complexes based on Pt(iv), Ru(ii) and Ru(iii) either as anticancer drugs or as molecular (photo)reagents for biomedical applications. The DNA binding mechanisms of these compounds are highlighted alongside with the novel strategies developed to improve their reactivity and/or specificity towards DNA. | |||||
BibTeX:
@article{PUB0051,
author = {Moucheron, Cecile},
title = {From cisplatin to photoreactive Ru complexes: targeting DNA for biomedical applications},
journal = {New J. Chem.},
publisher = {The Royal Society of Chemistry},
year = {2009},
volume = {33},
pages = {235-245},
url = {http://dx.doi.org/10.1039/B817016A},
doi = {http://dx.doi.org/10.1039/B817016A}
}
|
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| Ghizdavu, L., Pierard, F., Rickling, S., Aury, S., Surin, M., Beljonne, D., Lazzaroni, R., Murat, P., Defrancq, E., Moucheron, C. and Kirsch-De Mesmaeker, A. | Oxidizing Ru(II) Complexes as Irreversible and Specific Photo-Cross-Linking Agents of Oligonucleotide Duplexes | 2009 | Inorg. Chem. Vol. 48(23), pp. 10988-10994 |
article | DOI URL |
| Abstract: Oxidizing polyazaaromatic RuII complexes containing two TAP ligands (TAP = 1,4,5,8-tetraazaphenanthrene) are able under illumination to cross-link irreversibly the two strands of an oligonucleotide (ODN) duplex by covalent bond formation. The cross-linking proceeds by two successive absorptions of a photon. An adduct of the metallic complex on a guanine (G) base of one ODN strand is first photoproduced, followed by a second photoaddition of the same Ru species to a G base of the complementary strand, provided that the two G moieties are separated by 0 or 1 base pair. These two processes lead to the cross-linking of the two strands. Such a photo-cross-linking is easily detected with [Ru(TAP)2(phen)]2+ (1; phen = 1,10-phenanthroline) and [Ru(HAT)2(phen)]2+ (2; HAT = 1,4,5,8,9,12-hexaazatriphenylene), whereas it is not observed with [Ru(TAP)2TPAC]2+ (3; TPAC = tetrapyridoacridine) at the same level of loading of the duplex by 3. With a concentration of 3 similar to that of 1 and 2, when the loading of the duplex by 3 is much more important than with 1 and 2, the photo-cross-linking with 3 can thus also be observed. As 3 intercalates its TPAC ligand into the base pairs stack, its mobility is restricted in the duplex. In contrast, 1 and 2 can adopt different geometries of interaction, which probably facilitate the photo-cross-linking. | |||||
BibTeX:
@article{PUB0160,
author = {Ghizdavu, Liana and Pierard, Frédéric and Rickling, Stéphane and Aury, Sabrina and Surin, Mathieu and Beljonne, David and Lazzaroni, Roberto and Murat, Pierre and Defrancq, Eric and Moucheron, Cécile and Kirsch-De Mesmaeker, Andrée},
title = {Oxidizing Ru(II) Complexes as Irreversible and Specific Photo-Cross-Linking Agents of Oligonucleotide Duplexes},
journal = {Inorg. Chem.},
year = {2009},
volume = {48},
number = {23},
pages = {10988-10994},
url = {http://pubs.acs.org/doi/abs/10.1021/ic901007w},
doi = {http://dx.doi.org/10.1021/ic901007w}
}
|
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| Perrier, S., Mugeniwabagara, E., Kirsch-De Mesmaeker, A., Hore, P.J. and Luhmer, M. | Exploring Photoreactions between Polyazaaromatic Ru(II) Complexes and Biomolecules by Chemically Induced Dynamic Nuclear Polarization Measurements | 2009 | J. Am. Chem. Soc. Vol. 131(34), pp. 12458-12465 |
article | DOI |
| Abstract: Steady-state 1H photo-chemically induced dynamic nuclear polarization (CIDNP) experiments were conducted at 14.1 T on deoxygenated (buffered pH 7) aqueous solutions of [Ru(phen)3]2+, [Ru(tap)2(phen)]2+, and [Ru(tap)3]2+ (tap = 1,4,5,8-tetraazaphenanthrene; phen = 1,10-phenanthroline) in the presence of guanosine-5′-monophosphate or N-acetyltyrosine. For the first time, CIDNP arising from photo-oxidation by polyazaaromatic Ru(II) complexes is reported. In agreement with the occurrence of a photo-electron-transfer process, photo-CIDNP effects are observed with [Ru(tap)3]2+ and [Ru(tap)2(phen)]2+ but not with [Ru(phen)3]2+. With [Ru(tap)2(phen)]2+, no significant photo-CIDNP is observed for the 1H nuclei of the phen ligand, consistent with the fact that the metal-to-ligand charge-transfer triplet excited states responsible for the photo-oxidation involve a tap ligand. Successive experiments with [Ru(tap)3]2+ highlight the accumulation of long-lived radical species in solution that cause 1H NMR signal broadening and photo-CIDNP extinction. The 1H photo-CIDNP observed for the biomolecules is rather weak, less than about 30% of the equilibrium magnetization. However, up to 60% polarization enhancement is observed for H-2 and H-7 of the tap ligands, which indicates high unpaired electron density in the vicinity of these atoms in the transient radical pair. This is consistent with the structure of known photoadducts formed, for instance, between the metallic compounds and the guanine base of mono- and polynucleotides. Indeed, in these adducts the covalent bond involves carbon C-2 or C-7 of a tap ligand. The occurrence of photo-CIDNP with polyazaaromatic Ru(II) complexes opens new perspectives for the study of this type of compound. | |||||
BibTeX:
@article{PUB0401,
author = {Perrier, Sandrine and Mugeniwabagara, Epiphanie and Kirsch-De Mesmaeker, Andrée and Hore, P. J. and Luhmer, Michel},
title = {Exploring Photoreactions between Polyazaaromatic Ru(II) Complexes and Biomolecules by Chemically Induced Dynamic Nuclear Polarization Measurements},
journal = {J. Am. Chem. Soc.},
year = {2009},
volume = {131},
number = {34},
pages = {12458-12465},
doi = {http://dx.doi.org/10.1021/ja9024287}
}
|
|||||
| Kovbasyuk, L., Moucheron, C., Dubois, P. and Kirsch-De Mesmaeker, A. | Probing the interaction between DNA and cell transfection polymers with luminescent RuIIcomplexes | 2009 | New J. Chem. Vol. 33, pp. 1047-1056 |
article | DOI URL |
| Abstract: The luminescence properties of RuII complexes [Ru(TAP)2(phen)]Cl2 (1), [Ru(TAP)2(TPAC)]Cl2 (2) and [Ru(phen)2(TPAC)]Cl2 (3) (TAP = 1,4,5,8-tetraazaphenanthrene and TPAC = tetrapyrido-acridine) were used for probing the interactions of cell transfection (co)polymers, such as poly[2-dimethylaminoethyl-methacrylate] (PDMAEMA) and poly[2-dimethylaminoethyl-methacrylate]-b-poly[(ethylene glycol-[small alpha]-methylether-[small omega]-methacrylate] (P(DMAEMA-b-MAPEG)), with different polynucleotides (poly[(dA-dT)]2 and poly[(dG-dC)]2) and nucleic acids (calf thymus DNA and pBR322 plasmid DNA). It turned out that 1 was not a useful probe because its affinity constant for DNA was too weak. Moreover, 3 was also excluded because DNA-Ru complex aggregates complicated the interpretation of the data. 2 was the only candidate that could be used as a luminescent probe of "DNA-polymer" self-assemblies that would penetrate cell membranes. Different (co)polymer/polynucleotide ratios were tested with complex 2 by luminescence measurements. The results showed that 2 is an interesting probe, which is very sensitive to changes in the polynucleotide double helix structure that are induced by interactions with the synthetic (co)polymer. Moreover, complex 2 has a mode of action of emission that is different from that of the classically used ethidium bromide, i.e. an increase in emission when certain "polymer/DNA" ratios are reached during the titration of DNA with the polymer. | |||||
BibTeX:
@article{PUB0542,
author = {Kovbasyuk, Larisa and Moucheron, Cecile and Dubois, Philippe and Kirsch-De Mesmaeker, Andrée},
title = {Probing the interaction between DNA and cell transfection polymers with luminescent RuIIcomplexes},
journal = {New J. Chem.},
year = {2009},
volume = {33},
pages = {1047-1056},
url = {http://dx.doi.org/10.1039/B815193H},
doi = {http://dx.doi.org/10.1039/B815193H}
}
|
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